[paper] Device for the Detection and Quantification of Exosomes

Diego Barrettino1, Christoph Zumbühl1, Raphael Kummer1, Markus Thalmann1, Carolina Balbi2, Giuseppe Vassalli2, Rosane Moura Dos Santos3 and Jean-Michel Sallese3

Low-Cost Portable Medical Device for the Detection and Quantification of Exosomes

2023 IEEE SENSORS, Vienna, Austria, 2023, pp. 1-4,

doi: 10.1109/SENSORS56945.2023.10325271. 

1 Lucerne University of Applied Sciences and Arts (HSLU), Institute of Electrical Engineering, CH- 6048 Horw, Switzerland
2 Istituto Cardiocentro Ticino, Via Tesserete 48, CH-6900 Lugano, Switzerland
3 Swiss Federal Institute of Technology Lausanne (EPFL), EPFL SCI STI EDLAB, Station 11, CH-1015 Lausanne, Switzerland

Abstract: The design, fabrication and testing of a low-cost portable medical device for the detection and quantification of exosomes is presented in this paper. The portable medical device comprises a sensor array that can detect the presence of exosomes and quantify its concentration, a microfluidic device that handles the human serum containing the exosomes, and all the necessary readout and control electronics. Measurement results performed with exosomes showed that the portable medical device can detect exosomes with a concentration of 2.5x108 /µL thus paving the way to a wide range of diagnostic applications.

Fig. Exosome immobilized on top of the functionalized (antibody CD63 +protein G
+ polydopamine + graphene oxide) JLFET biosensor and tagged with a gold (Au) nanoparticle attached to an exosome biomarker (antibody CD31)